Architecture of eukaryotic mRNA 3′-end processing machinery
نویسندگان
چکیده
منابع مشابه
Architecture of eukaryotic mRNA 3′-end processing machinery
Newly transcribed eukaryotic precursor messenger RNAs (pre-mRNAs) are processed at their 3' ends by the ~1-megadalton multiprotein cleavage and polyadenylation factor (CPF). CPF cleaves pre-mRNAs, adds a polyadenylate tail, and triggers transcription termination, but it is unclear how its various enzymes are coordinated and assembled. Here, we show that the nuclease, polymerase, and phosphatase...
متن کاملMolecular mechanisms of eukaryotic pre-mRNA 3′ end processing regulation
Messenger RNA (mRNA) 3' end formation is a nuclear process through which all eukaryotic primary transcripts are endonucleolytically cleaved and most of them acquire a poly(A) tail. This process, which consists in the recognition of defined poly(A) signals of the pre-mRNAs by a large cleavage/polyadenylation machinery, plays a critical role in gene expression. Indeed, the poly(A) tail of a matur...
متن کاملDelineating the structural blueprint of the pre-mRNA 3'-end processing machinery.
Processing of mRNA precursors (pre-mRNAs) by polyadenylation is an essential step in gene expression. Polyadenylation consists of two steps, cleavage and poly(A) synthesis, and requires multiple cis elements in the pre-mRNA and a megadalton protein complex bearing the two essential enzymatic activities. While genetic and biochemical studies remain the major approaches in characterizing these fa...
متن کاملEukaryotic mRNA 3' processing: a common means to different ends.
In principle, the formation of the 3 end of a eukaryotic mRNA is a simple process. At a minimum, it involves the hydrolysis of a single phosphodiester bond in the nascent transcript. For one unique class of mRNAs, transcripts of the metazoan replication-dependent histone genes, this is indeed the only requirement. All other eukaryotic mRNAs require both cleavage and subsequent poly(A) addition ...
متن کاملConnections between mRNA 3' end processing and transcription termination.
Discoveries within the last few years have revealed that the multiple steps in gene expression are remarkably integrated. There have recently been several advances in deciphering how mRNA 3' end processing is linked with transcription elongation and termination. It has been known for quite a long time that transcription termination is somehow intertwined with polyadenylation, but it is still un...
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ژورنال
عنوان ژورنال: Science
سال: 2017
ISSN: 0036-8075,1095-9203
DOI: 10.1126/science.aao6535